Journal: Advanced Science

Article Title: Adipocyte‐Derived Exosomal MTTP Suppresses Ferroptosis and Promotes Chemoresistance in Colorectal Cancer

doi: 10.1002/advs.202203357

Figure Lengend Snippet: Adipocyte exosomes reduce the sensitivity of CRC organoids to L‐OHP: A) Schematic description of the organoids development. B) Representative images of organoids growth were recorded by bright‐field microscope. Scale bar = 100 µm. C) Multiplex Fluorescent Immunohistochemistry Staining images of organoids expressing specific markers CD44 (red), Ki67 (pink), CEA (yellow) and EP‐CAM (green) with blue DAPI staining (scale bar = 500 µm). D) Immunohistochemistry analysis of CD44, Ki67, CEA and EP‐CAM levels in serial organoids sections (scale bar = 500 µm). E) Cell viability assay detection of the viability of oxaliplatin‐exposed organoids. F) Representative images of organoids treated with L‐OHP, pretreated with MTTP siRNA, exosomes secreted by KD‐MTTP adipocytes or mature adipocyte exosomes and then treated with oxaliplatin (scale bar = 200 µm). G) Cell viability of organoids described in (F). Data are presented as means ± SD of three simultaneously performed experiments (G). P value was calculated using one‐way ANOVA; n.s., not significant; * P < 0.05, ** P < 0.01, *** P < 0.001.

Article Snippet: The following primary antibodies and subsequent Opal TSA combinations were used: anti‐CD44 (1:250, abcam, ab254530) and Opal 520, anti‐ki67 (1:250, abcam, ab92742) and Opal 570, anti‐CEA (1:400, bioss, bs‐0719R) and Opal 620, anti‐EP‐CAM (1:250, Santa Cruz, sc‐53532) and Opal 690, and DAPI.

Techniques: Microscopy, Multiplex Assay, Immunohistochemistry, Staining, Expressing, Viability Assay

Journal:

Article Title: NCAM(CD56) and RUNX1(AML1) Are Up-Regulated in Human Ischemic Cardiomyopathy and a Rat Model of Chronic Cardiac Ischemia

doi:

Figure Lengend Snippet: First and Second Antibodies Applied in this Study

Article Snippet: C-CAM1 (CD66), Santa Cruz (N-19), F , 1:400.

Techniques: Concentration Assay

Journal: Advanced Science

Article Title: Adipocyte‐Derived Exosomal MTTP Suppresses Ferroptosis and Promotes Chemoresistance in Colorectal Cancer

doi: 10.1002/advs.202203357

Figure Lengend Snippet: Adipocyte exosomes reduce the sensitivity of CRC organoids to L‐OHP: A) Schematic description of the organoids development. B) Representative images of organoids growth were recorded by bright‐field microscope. Scale bar = 100 µm. C) Multiplex Fluorescent Immunohistochemistry Staining images of organoids expressing specific markers CD44 (red), Ki67 (pink), CEA (yellow) and EP‐CAM (green) with blue DAPI staining (scale bar = 500 µm). D) Immunohistochemistry analysis of CD44, Ki67, CEA and EP‐CAM levels in serial organoids sections (scale bar = 500 µm). E) Cell viability assay detection of the viability of oxaliplatin‐exposed organoids. F) Representative images of organoids treated with L‐OHP, pretreated with MTTP siRNA, exosomes secreted by KD‐MTTP adipocytes or mature adipocyte exosomes and then treated with oxaliplatin (scale bar = 200 µm). G) Cell viability of organoids described in (F). Data are presented as means ± SD of three simultaneously performed experiments (G). P value was calculated using one‐way ANOVA; n.s., not significant; * P < 0.05, ** P < 0.01, *** P < 0.001.

Article Snippet: The following primary antibodies and subsequent Opal TSA combinations were used: anti‐CD44 (1:250, abcam, ab254530) and Opal 520, anti‐ki67 (1:250, abcam, ab92742) and Opal 570, anti‐CEA (1:400, bioss, bs‐0719R) and Opal 620, anti‐EP‐CAM (1:250, Santa Cruz, sc‐53532) and Opal 690, and DAPI.

Techniques: Microscopy, Multiplex Assay, Immunohistochemistry, Staining, Expressing, Viability Assay

Journal: Advanced Science

Article Title: Adipocyte‐Derived Exosomal MTTP Suppresses Ferroptosis and Promotes Chemoresistance in Colorectal Cancer

doi: 10.1002/advs.202203357

Figure Lengend Snippet: Effects of adipocyte exosomes on colorectal carcinoma in a murine model: A) Schematic description of the experimental design for the in vivo models. B,C) Photos of mice and tumors from all groups. D) Anatomical location of abdominal fat pad in ob/ob mouce. E) In vivo fluorescence signals were recorded by IVIS SPECTRUM. Fluorescence was detected at the abdominal fat pad. Mouse weights F), tumor volumes G) and tumor weights H) in each group ( n = 5). I) IHC analysis of MTTP levels in abdominal fat pad in each group ( n = 5). Scale bar = 50 µm. J) WB analysis of MTTP protein levels in abdominal fat pad in each group and quantified using a gray scale analysis. K) Expression of ZEB1, MTTP, GPX4 and xCT detected by western blot and quantified using a gray scale analysis ( n = 5). L) IHC analysis of GPX4, xCT, ki67, and MTTP levels of tumors in each group ( n = 5). Scale bar = 50 µm. Data are presented as means ± SD of three simultaneously performed experiments J,K). P value was calculated using one‐way ANOVA; * P < 0.05, ** P < 0.01, *** P < 0.001.

Article Snippet: The following primary antibodies and subsequent Opal TSA combinations were used: anti‐CD44 (1:250, abcam, ab254530) and Opal 520, anti‐ki67 (1:250, abcam, ab92742) and Opal 570, anti‐CEA (1:400, bioss, bs‐0719R) and Opal 620, anti‐EP‐CAM (1:250, Santa Cruz, sc‐53532) and Opal 690, and DAPI.

Techniques: In Vivo, Fluorescence, Expressing, Western Blot

Journal: Advanced Science

Article Title: Adipocyte‐Derived Exosomal MTTP Suppresses Ferroptosis and Promotes Chemoresistance in Colorectal Cancer

doi: 10.1002/advs.202203357

Figure Lengend Snippet: Effects of adipocyte exosomes on colorectal carcinoma in a murine model: A) Schematic description of the experimental design for the in vivo models. B,C) Photos of mice and tumors from all groups. D) Numbers of liver metastases in each group ( n = 5). Mouse weights E), tumor weights F), and tumor volumes G) in each group ( n = 5). WB analysis of ZEB1, MTTP, GPX4, and xCT protein levels in tumors H) and quantified by a gray scale analysis I). J) Expression of MTTP, GPX4, xCT, and ki67 detected using IHC ( n = 5). Data are presented as means ± SD of three simultaneously performed experiments (G,I). P value was calculated using one‐way ANOVA; * P < 0.05, ** P < 0.01, *** P < 0.001.

Article Snippet: The following primary antibodies and subsequent Opal TSA combinations were used: anti‐CD44 (1:250, abcam, ab254530) and Opal 520, anti‐ki67 (1:250, abcam, ab92742) and Opal 570, anti‐CEA (1:400, bioss, bs‐0719R) and Opal 620, anti‐EP‐CAM (1:250, Santa Cruz, sc‐53532) and Opal 690, and DAPI.

Techniques: In Vivo, Expressing